KRIT1 antibody (C-Term)

Catalog No. ABIN4886652

This anti-KRIT1 antibody is a Rabbit Polyclonal antibody detecting KRIT1 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for KRIT1 antibody (C-Term) (ABIN4886652)

Target: KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRIT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KRIT1 Antibody

Binding Specificity: AA 703-736, C-Term

Purpose: Anti-KRIT1 Antibody Picoband®

Sequence: ENKMSFIVHT KQAGLVVKLL MKLNGQLMPT ERNS

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KRIT1 Antibody Picoband® (ABIN4886652). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human KRIT1, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Eerola I, McIntyre B, Vikkula M (Feb 2001). "Identification of eight novel 5'-exons in cerebral capillary malformation gene-1 (CCM1) encoding KRIT1".Biochimica et Biophysica Acta 1517 (3): 464-7. 2. Pagenstecher A, Stahl S, Sure U, Felbor U (Mar 2009)."A two-hit mechanism causes cerebral cavernous malformations: complete inactivation of CCM1, CCM2 or CCM3 in affected endothelial cells". Human Molecular Genetics 18 (5): 911-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for KRIT1

Target: KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))

Alternative Name: KRIT1

Background:

Synonyms: Krev interaction trapped protein 1,Krev interaction trapped 1,Cerebral cavernous malformations 1 protein,KRIT1,CCM1,

Tissue Specificity: Low levels in brain. Very weak expression found in heart and muscle. .

Background: Krev interaction trapped protein 1 (KRIT1) is a protein that in humans is encoded by the CCM1 gene. This gene encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 84 kDa

Gene ID: 889

UniProt: O00522

Pathways: Cell RedoxHomeostasis