Cytokeratin 5 antibody (Middle Region)

Catalog No. ABIN4886654

The Rabbit Polyclonal anti-Cytokeratin 5 antibody is suitable to detect Cytokeratin 5 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Cytokeratin 5 antibody (Middle Region) (ABIN4886654)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cytokeratin 5 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Cytokeratin 5/KRT5 Antibody

Sequence: KVELEAKVDA LMDEINFMKM FFDAELSQMQ TH

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by one amino acid,and identical to the related rat sequence.

Characteristics: Anti-Cytokeratin 5/KRT5 Antibody. Tested in IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: KRT5

Background:

Background: Cytokeratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Gene Full Name: keratin 5

Molecular Weight: 62 kDa

Gene ID: 3852

UniProt: P13647