LDHB antibody (AA 237-334)

Catalog No. ABIN4886658

The Rabbit Polyclonal anti-LDHB antibody (ABIN4886658) specifically detects LDHB in WB, IHC, ICC, IF and FACS. The antibody is reactive with Human, Rat, Mouse and Monkey samples.

Quick Overview for LDHB antibody (AA 237-334) (ABIN4886658)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-LDHB Antibody

Binding Specificity: AA 237-334

Purpose: Anti-Lactate Dehydrogenase B/LDHB Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse,rat LDHB.

Characteristics: Anti-Lactate Dehydrogenase B/LDHB Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDHB

Background:

Background: The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

Gene Full Name: lactate dehydrogenase B

Molecular Weight: 37 kDa

Gene ID: 3945

UniProt: P07195

Pathways: Warburg Effect