MAP3K8 antibody (AA 9-193)

Catalog No. ABIN4886663

The Rabbit Polyclonal anti-MAP3K8 antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect MAP3K8 in samples from Human and Rat.

Quick Overview for MAP3K8 antibody (AA 9-193) (ABIN4886663)

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MAP3K8 Antibody

Binding Specificity: AA 9-193

Purpose: Anti-MAP3K8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MAP3K8 Antibody Picoband® (ABIN4886663). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAP3K8 recombinant protein (Position: D9-L193). Human MAP3K8 shares 89.7% and 90.3% amino acid (aa) sequence identity with mouse and rat MAP3K8, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: MAP3K8 mitogen-activated protein kinase kinase kinase 8". 2. Chan AM, Chedid M, McGovern ES, Popescu NC, Miki T, Aaronson SA (May 1993). "Expression cDNA cloning of a serine kinase transforming gene". Oncogene 8 (5): 1329-33. 3. Miyoshi J, Higashi T, Mukai H, Ohuchi T, Kakunaga T (Aug 1991). "Structure and transforming potential of the human cot oncogene encoding a putative protein kinase". Molecular and Cellular Biology 11 (8): 4088-96.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAP3K8

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Alternative Name: MAP3K8

Background:

Synonyms: Mitogen-activated protein kinase kinase kinase 8,2.7.11.25,Cancer Osaka thyroid oncogene,Proto-oncogene c-Cot,Serine/threonine-protein kinase cot,Tumor progression locus 2,TPL-2,MAP3K8,COT, ESTF,

Tissue Specificity: Expressed in several normal tissues and human tumor-derived cell lines.

Background: Mitogen-activated protein kinase kinase kinase 8 is an enzyme that in humans is encoded by the MAP3K8 gene. This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. Additionally, this protein was found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molecular Weight: 53 kDa

Gene ID: 1326

UniProt: P41279

Pathways: PI3K-Akt Signaling, TCR Signaling