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NAMPT antibody (Nicotinamide phosphoribosyltransferase) (AA 62-337) Primary Antibody

NAMPT Reactivity: Human, Mouse, Rat ELISA, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN4886677
$280.00
Plus shipping costs $45.00
100 μg
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  • Target
    NAMPT
    Binding Specificity
    AA 62-337
    • 26
    • 22
    • 15
    • 8
    • 8
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Reactivity
    Human, Mouse, Rat
    • 154
    • 71
    • 71
    • 8
    • 8
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    Host
    Rabbit
    • 143
    • 19
    • 6
    • 4
    • 1
    Clonality
    Polyclonal
    • 155
    • 19
    Conjugate
    This NAMPT antibody is un-conjugated
    • 79
    • 27
    • 12
    • 6
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Application
    ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    • 103
    • 87
    • 58
    • 24
    • 23
    • 22
    • 16
    • 16
    • 14
    • 9
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Purpose
    Rabbit IgG polyclonal antibody for Nicotinamide phosphoribosyltransferase(NAMPT) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Nicotinamide phosphoribosyltransferase(NAMPT) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
    Gene Name: nicotinamide phosphoribosyltransferase
    Protein Name: Nicotinamide phosphoribosyltransferase
    Purification
    Immunogen affinity purified.
    Immunogen
    E. coli-derived human Visfatin recombinant protein (Position: L62-N337). Human Visfatin shares 97.1% and 96.7% amino acid (aa) sequence identity with mouse and rat Visfatin, respectively.
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Target
    NAMPT
    Alternative Name
    NAMPT (NAMPT Antibody Abstract)
    Synonyms
    1110035O14Rik, PBEF, PBEF1, VF, VISFATIN, AI314458, AI480535, NAmPRTase, Pbef, Pbef1, Visfatin, visfatin, pbef, pbef1, nicotinamide phosphoribosyltransferase, Nicotinamide phosphoribosyltransferase, nicotinamide phosphoribosyltransferase S homeolog, NAMPT, Nampt, nampt, Smon_0680, Mrub_2845, Cseg_3704, BC1002_3997, Ftrac_2686, Varpa_5681, Celal_3900, Deima_0880, BC1001_3724, Celly_1696, Clole_1542, Tsp_05934, nampt.S
    Background
    Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.

    Synonyms: NAmPRTase | Nampt | PBEF 1 | PBEF1 | PBEF | VF | Visfatin | P43490
    Gene ID
    10135
    UniProt
    P43490
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