NAMPT antibody (Nicotinamide phosphoribosyltransferase) (AA 62-337)

Details for Product anti-NAMPT Antibody No. ABIN4886677
Antigen
  • 1110035O14Rik
  • PBEF
  • PBEF1
  • VF
  • VISFATIN
  • AI314458
  • AI480535
  • NAmPRTase
  • Pbef
  • Pbef1
  • Visfatin
  • visfatin
  • pbef
  • pbef1
  • nicotinamide phosphoribosyltransferase
  • Nicotinamide phosphoribosyltransferase
  • nicotinamide phosphoribosyltransferase S homeolog
  • NAMPT
  • Nampt
  • nampt
  • Smon_0680
  • Mrub_2845
  • Cseg_3704
  • BC1002_3997
  • Ftrac_2686
  • Varpa_5681
  • Celal_3900
  • Deima_0880
  • BC1001_3724
  • Celly_1696
  • Clole_1542
  • Tsp_05934
  • nampt.S
Epitope
AA 62-337
29
25
15
12
10
9
8
8
8
7
6
6
6
6
6
5
5
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
278
127
122
11
10
10
8
8
7
6
5
4
3
1
Host
Rabbit
269
47
6
6
2
Clonality
Polyclonal
Conjugate
This NAMPT antibody is un-conjugated
44
14
9
6
6
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
1
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
231
188
108
31
29
24
24
20
20
12
10
9
4
3
2
1
1
1
1
Options
Purpose Rabbit IgG polyclonal antibody for Nicotinamide phosphoribosyltransferase(NAMPT) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Immunogen E. coli-derived human Visfatin recombinant protein (Position: L62-N337). Human Visfatin shares 97.1% and 96.7% amino acid (aa) sequence identity with mouse and rat Visfatin, respectively.
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Nicotinamide phosphoribosyltransferase(NAMPT) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Gene Name: nicotinamide phosphoribosyltransferase
Protein Name: Nicotinamide phosphoribosyltransferase
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others NAMPT products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name NAMPT (NAMPT Antibody Abstract)
Background Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.

Synonyms: NAmPRTase | Nampt | PBEF 1 | PBEF1 | PBEF | VF | Visfatin | P43490
Gene ID 10135
UniProt P43490
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Supplier Images
Image no. 1 for anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 62-337) antibody (ABIN4886677) Visfatin was detected in paraffin-embedded sections of rat intestine tissues using ra...
Image no. 2 for anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 62-337) antibody (ABIN4886677) Western blot analysis of Visfatin expression in rat brain extract ( Lane 1), mouse ca...
Image no. 3 for anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 62-337) antibody (ABIN4886677) Visfatin was detected in paraffin-embedded sections of human mammary cancer tissues u...
Image no. 4 for anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 62-337) antibody (ABIN4886677) Visfatin was detected in paraffin-embedded sections of mouse intestine tissues using ...
Did you look for something else?