PNP antibody (Middle Region)

Catalog No. ABIN4886701

This Rabbit Polyclonal antibody specifically detects PNP in WB, IHC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for PNP antibody (Middle Region) (ABIN4886701)

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PNP Antibody

Binding Specificity: Middle Region

Purpose: Anti-Nucleoside phosphorylase/PNP Antibody

Sequence: AMSDAYDRTM RQRALSTWKQ MGEQRELQE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by six amino acids,and from the related rat sequence by five amino acids.

Characteristics: Anti-Nucleoside phosphorylase/PNP Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PNP, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PNP

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Alternative Name: PNP

Background:

Background: The PNP gene encodes purine nucleoside phosphorylase, an enzyme that catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. It is presented results from gene dosage studies consistent with assignment of the PNP locus to band 14q13. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised Tlymphocyte function and neurologic dysfunction.

Gene Full Name: purine nucleoside phosphorylase

Molecular Weight: 32 kDa

Gene ID: 4860

UniProt: P00491

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Ribonucleoside Biosynthetic Process, Positive Regulation of Response to DNA Damage Stimulus