PSMA2 antibody (Middle Region)

Catalog No. ABIN4886705

The Rabbit Polyclonal anti-PSMA2 antibody has been validated for WB. It is suitable to detect PSMA2 in samples from Human and Rat.

Quick Overview for PSMA2 antibody (Middle Region) (ABIN4886705)

Target: PSMA2 (Proteasome Subunit alpha 2 (PSMA2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMA2 Antibody

Binding Specificity: AA 82-123, Middle Region

Purpose: Anti-Proteasome 20S alpha 2/PSMA2 Antibody Picoband®

Sequence: DYRVLVHRAR KLAQQYYLVY QEPIPTAQLV QRVASVMQEY T

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Proteasome 20S alpha 2/PSMA2 Antibody Picoband® (ABIN4886705). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PSMA2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Groll M, Ditzel L, Löwe J, Stock D, Bochtler M, Bartunik HD, Huber R (Apr 1997). "Structure of 20S proteasome from yeast at 2.4 A resolution". Nature 386 (6624): 463-71. 2. Tomko RJ, Hochstrasser M (2013). "Molecular architecture and assembly of the eukaryotic proteasome". Annual Review of Biochemistry 82: 415-45.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PSMA2

Target: PSMA2 (Proteasome Subunit alpha 2 (PSMA2))

Alternative Name: PSMA2

Background:

Synonyms: Proteasome subunit alpha type-2,3.4.25.1,Macropain subunit C3,Multicatalytic endopeptidase complex subunit C3,Proteasome component C3,PSMA2,HC3, PSC3,

Tissue Specificity: Expressed at high levels in the brain, liver, kidney and pancreas, and very weakly expressed in the thymus, prostate, testis and colon.

Background: Proteasome subunit alpha type-2 is a protein that in humans is encoded by the PSMA2 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Molecular Weight: 26 kDa

Gene ID: 5683

UniProt: P25787

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA