TUBB antibody (C-Term)

Catalog No. ABIN4886757

This anti-TUBB antibody is a Rabbit Polyclonal antibody detecting TUBB in WB, IHC, IF, FACS and ICC. Suitable for Human, Mouse, Rat, Chicken and Monkey. This Primary Antibody has been cited in 3+ publications.

Quick Overview for TUBB antibody (C-Term) (ABIN4886757)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Chicken, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-TUBB Antibody

Binding Specificity: AA 383-412, C-Term

Purpose: Anti-Beta Tubulin/TUBB Antibody Picoband®

Sequence: EQFTAMFRRK AFLHWYTGEG MDEMEFTEAE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Beta Tubulin/TUBB Antibody Picoband® (ABIN4886757). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: TUBB tubulin, beta". 2. Volz A, Weiss E, Trowsdale J, Ziegler A (Jan 1994). "Presence of an expressed beta-tubulin gene (TUBB) in the HLA class I region may provide the genetic basis for HLA-linked microtubule dysfunction". Human Genetics. 93 (1): 42-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-TUBB antibody (ABIN4886757)

Qu, Zhang, Du, Wang, Yang, Guo, Wang, Zhang, Xu: "Pim-3 is a Critical Risk Factor in Development and Prognosis of Prostate Cancer." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 22, pp. 4254-4260, (2017) (PubMed).

Zhu, Liu, Wang, Nie, He, Zhang, Liu, Su: "Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5691-700, (2016) (PubMed).

Cao, Li, Li, Xiong, Zhou, Fan, Yu, Mao: "The potential role of HMGB1 release in peritoneal dialysis-related peritonitis." in: PLoS ONE, Vol. 8, Issue 1, pp. e54647, (2013) (PubMed).

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: TUBB

Background:

Synonyms: Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5, OK/SW-cl.56

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Molecular Weight: 50 kDa

Gene ID: 203068

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase