Acetylated Lysine antibody (acetylated, Lys25)

Catalog No. ABIN488677

The Mouse Monoclonal anti-Acetylated Lysine antibody is suitable to detect Acetylated Lysine in samples from Cow and Avian. It has been validated for WB, ELISA, IHC, IP, IF and ICC.

Quick Overview for Acetylated Lysine antibody (acetylated, Lys25) (ABIN488677)

Target: Acetylated Lysine

Reactivity: Cow, Avian

Host: Mouse

Clonality: Monoclonal

Conjugate: This Acetylated Lysine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 7F8

Product Details anti-Acetylated Lysine Antibody

Binding Specificity: acetylated, Lys25

Purpose: Anti-Acetylated lysine (25 μl, clone 7F8)

Specificity: The antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.

Cross-Reactivity (Details): Bovine, avian
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Higher plants

Purification: Protein G purified

Immunogen: acetylated KLH

Isotype: IgG1

Application Details

Application Notes: 1 : 100 (IHC), 1 : 1000 (WB)

Comment:

1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Total IgG fraction. Protein G purified.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for Acetylated Lysine

Target: Acetylated Lysine

Target Type: Chemical

Background:

Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).

Additional information: Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/mLantibody detects Proteins containing acetylated lysine residues in ELISA and WBs, Does not detect non-acetylated lysine residues