Biotin antibody

Catalog No. ABIN4889010

The Mouse Monoclonal anti-Biotin antibody (Clone 15D9) (ABIN4889010) specifically detects Biotin in WB, IHC and ELISA.

Quick Overview for Biotin antibody (ABIN4889010)

Target: Biotin

Host: Mouse

Clonality: Monoclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 15D9

Product Details anti-Biotin Antibody

Specificity: Reacts with free biotin and biotin bounds on carrier protein

Purification: Purified (protein A)

Immunogen: Biotin

Isotype: IgG2b kappa

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA(1:5000 - 1:10000) IHC(1:200 - 1:2000) WB(1:1000 - 1:5000)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M, sucrose 2 %

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Biotin is a tag used in biological reactions for its affinity to avidin or streptavidin.