N-epsilon-gamma-Glutamyl-Lysine Isopeptide antibody
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- Target
- N-epsilon-gamma-Glutamyl-Lysine Isopeptide
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Reactivity
- All Species
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificity
- Reacts with Ne (g-L-glutamyl)-L-lysine
- Cross-Reactivity (Details)
- Cross reacts in ELISA with N1,N4 bis (gPoly L-glutamyl) Putrescine and N1,N8 bis (gPoly L-glutamyl) Spermidine. No cross reaction with : - protein bound Ne acetyl lysine - protein bound N1 or N4 mono (gPoly L-glutamyl) Putrescine - protein bound N1 mono (gPoly L-glutamyl) Spermidine - protein bound Ne (g -L-glutamyl) lysine having a free alpha amine either on its glutamyl or lysyl moieties. Ability to fix complement : unknown
- Purification
- Purified
- Immunogen
- Nepsilon-(gamma-L-glutamyl)-L-lysine isopeptide
- Clone
- 81D4
- Isotype
- IgM kappa
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- Application Notes
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Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
IHC(1:50 - 1:800) - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Must be reconstituted in distilled water.
- Concentration
- 1 mg/mL
- Buffer
- PO4 20 mM, NaCl 1M, sucrose 2 %
- Storage
- 4 °C,-20 °C
- Storage Comment
- Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.
- Expiry Date
- 24 months
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- Target
- N-epsilon-gamma-Glutamyl-Lysine Isopeptide
- Alternative Name
- Nepsilon-(Gamma-L-Glutamyl)-L-Lysine Isopeptide
- Background
- Isopeptide bond are formed on lateral chains of proteins. Nε-(γ-L-glutamyl)-L-lysine isopeptide is generated after action of transglutaminase enzyme.
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