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N-epsilon-gamma-Glutamyl-Lysine Isopeptide antibody

Reactivity: All Species ELISA, IHC, IF Host: Mouse Monoclonal 81D4 unconjugated
Catalog No. ABIN4889369
  • Target
    N-epsilon-gamma-Glutamyl-Lysine Isopeptide
    Reactivity
    • 7
    • 2
    All Species
    Host
    • 9
    Mouse
    Clonality
    • 9
    Monoclonal
    Conjugate
    • 6
    • 2
    • 1
    Un-conjugated
    Application
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Specificity
    Reacts with Ne (g-L-glutamyl)-L-lysine
    Cross-Reactivity (Details)
    Cross reacts in ELISA with N1,N4 bis (gPoly L-glutamyl) Putrescine and N1,N8 bis (gPoly L-glutamyl) Spermidine. No cross reaction with : - protein bound Ne acetyl lysine - protein bound N1 or N4 mono (gPoly L-glutamyl) Putrescine - protein bound N1 mono (gPoly L-glutamyl) Spermidine - protein bound Ne (g -L-glutamyl) lysine having a free alpha amine either on its glutamyl or lysyl moieties. Ability to fix complement : unknown
    Purification
    Purified
    Immunogen
    Nepsilon-(gamma-L-glutamyl)-L-lysine isopeptide
    Clone
    81D4
    Isotype
    IgM kappa
  • Application Notes

    Working dilution: Optimal dilution should be determined by the end user.
    The following are guidelines only :
    IHC(1:50 - 1:800)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Must be reconstituted in distilled water.
    Concentration
    1 mg/mL
    Buffer
    PO4 20 mM, NaCl 1M, sucrose 2 %
    Storage
    4 °C,-20 °C
    Storage Comment
    Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.
    Expiry Date
    24 months
  • Target
    N-epsilon-gamma-Glutamyl-Lysine Isopeptide
    Alternative Name
    Nepsilon-(Gamma-L-Glutamyl)-L-Lysine Isopeptide
    Background
    Isopeptide bond are formed on lateral chains of proteins. Nε-(γ-L-glutamyl)-L-lysine isopeptide is generated after action of transglutaminase enzyme.
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