H2AFZ antibody (C-Term)

Catalog No. ABIN4889649

This Rabbit Polyclonal antibody specifically detects H2AFZ in WB, IF, ChIP, ChIP-seq and DB. It exhibits reactivity toward Human. It has been mentioned in 9+ publications

Quick Overview for H2AFZ antibody (C-Term) (ABIN4889649)

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFZ antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Dot Blot (DB)

Product Details anti-H2AFZ Antibody

Binding Specificity: C-Term

Purpose: Histone H2A.Z antibody (pAb) (H2AZ)

Purification: Unpurified

Immunogen: This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.

Application Details

Application Notes: ChIP: 2 - 10 µL per ChIP ChIP-Seq: 5 µL each WB*: 1:1,000 - 1:5,000 dilution This histone variant can be perturbed by excessive washing steps prior to fixation. Cells should be washed into serum-free culture medium (NOT PBS!) prior to fixation. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-H2AFZ antibody (ABIN4889649)

Law, Cheung: "Expression of Non-acetylatable H2A.Z in Myoblast Cells Blocks Myoblast Differentiation through Disruption of MyoD Expression." in: The Journal of biological chemistry, Vol. 290, Issue 21, pp. 13234-49, (2015) (PubMed).

Gemma, Ramagopalan, Down, Beyan, Hawa, Holland, Hurd, Giovannoni, Leslie, Ebers, Rakyan: "Inactive or moderately active human promoters are enriched for inter-individual epialleles." in: Genome biology, Vol. 14, Issue 5, pp. R43, (2014) (PubMed).

Menet, Pescatore, Rosbash: "CLOCK:BMAL1 is a pioneer-like transcription factor." in: Genes & development, Vol. 28, Issue 1, pp. 8-13, (2014) (PubMed).

Boyarchuk, Filipescu, Vassias, Cantaloube, Almouzni: "The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle." in: Journal of cell science, Vol. 127, Issue Pt 15, pp. 3347-59, (2014) (PubMed).

Lau, Cheung: "Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity." in: Nucleic acids research, Vol. 41, Issue 3, pp. e49, (2013) (PubMed).

Vierstra, Wang, John, Sandstrom, Stamatoyannopoulos: "Coupling transcription factor occupancy to nucleosome architecture with DNase-FLASH." in: Nature methods, Vol. 11, Issue 1, pp. 66-72, (2013) (PubMed).

Yu, Feng, Ruan, Komers, Kiviat, Bomsztyk: "Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays." in: BMC molecular biology, Vol. 12, pp. 49, (2011) (PubMed).

El Gazzar, Liu, Yoza, McCall: "Dynamic and selective nucleosome repositioning during endotoxin tolerance." in: The Journal of biological chemistry, Vol. 285, Issue 2, pp. 1259-71, (2010) (PubMed).

Conerly, Teves, Diolaiti, Ulrich, Eisenman, Henikoff: "Changes in H2A.Z occupancy and DNA methylation during B-cell lymphomagenesis." in: Genome research, Vol. 20, Issue 10, pp. 1383-90, (2010) (PubMed).

Target Details for H2AFZ

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Alternative Name: Histone H2A.Z

Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may enable H2A.Z to function as an insulator of chromatin domains.

Molecular Weight: 14 kDa

Gene ID: 3015

NCBI Accession: NP_002097

Pathways: Telomere Maintenance