Histone 3 antibody (H3K36me2)

Catalog No. ABIN4889654

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IF, ChIP, ICC, DB, ChIP-seq and CUT&Tag. It exhibits reactivity toward Human and Saccharomyces cerevisiae. It has been mentioned in 8+ publications

Quick Overview for Histone 3 antibody (H3K36me2) (ABIN4889654)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K36me2

Purpose: Histone H3K36me2 antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H3 dimethyl Lys36 antibody was raised against a peptide including dimethyl-lysine 36 of histone H3.

Application Details

Application Notes: ChIP: 3 - 5 µL per ChIP ChIP-Seq: 10 µL each ICC/IF: 1:250 - 1:1,000 dilution WB: 1:2500 - 1:5,000 dilution CUT&Tag: 1-2 µL per 50 µL reaction

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN4889654)

Young, Hillyer, Hokamp, Fitzpatrick, Konstantinov, Welty, Ness, Werner-Washburne, Fleming, Osley: "Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast." in: BMC genomics, Vol. 18, Issue 1, pp. 107, (2017) (PubMed).

García-Carpizo, Sarmentero, Han, Graña, Ruiz-Llorente, Pisano, Serrano, Brooks, Campbell, Barrero: "NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation." in: Scientific reports, Vol. 6, pp. 32952, (2016) (PubMed).

Luense, Denner, Fernández-Montalván, Hartung, Husemann, Stresemann, Prechtl: "Quantification of histone H3 Lys27 trimethylation (H3K27me3) by high-throughput microscopy enables cellular large-scale screening for small-molecule EZH2 inhibitors." in: Journal of biomolecular screening, Vol. 20, Issue 2, pp. 190-201, (2015) (PubMed).

Feng, Zhang, Shea, Creighton, Coarfa, Hilsenbeck, Lanz, He, Wang, Fu, Nardone, Song, Bradner, Mitsiades, Mitsiades, Osborne, Schiff, OMalley: "An epigenomic approach to therapy for tamoxifen-resistant breast cancer." in: Cell research, Vol. 24, Issue 7, pp. 809-19, (2014) (PubMed).

Jha, Strahl: "An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint activation and DSB repair." in: Nature communications, Vol. 5, pp. 3965, (2014) (PubMed).

Ma, Pan, Montgomery, Olson, Schultz: "Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 8, pp. E481-9, (2012) (PubMed).

Hainer, Martens: "Identification of histone mutants that are defective for transcription-coupled nucleosome occupancy." in: Molecular and cellular biology, Vol. 31, Issue 17, pp. 3557-68, (2011) (PubMed).

Hsia, Tepper, Pochampalli, Hsia, Izumiya, Huerta, Wright, Chen, Kung, Izumiya: "KDM8, a H3K36me2 histone demethylase that acts in the cyclin A1 coding region to regulate cancer cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 21, pp. 9671-6, (2010) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522