Histone 3 antibody (H3K27me3)

Catalog No. ABIN4889658

This Mouse Monoclonal antibody specifically detects Histone 3 in WB, IF, ChIP and ICC. It exhibits reactivity toward Human. It has been mentioned in 4+ publications

Quick Overview for Histone 3 antibody (H3K27me3) (ABIN4889658)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)

Clone: 7B11

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K27me3

Purpose: Histone H3K27me2me3 antibody (mAb) (Clone 7B11)

Purification: Protein A Chromatography

Immunogen: This Histone H3 di/trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 0.5 - 2 μg/mL dilution WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. This antibody has been published for use in ChIP, but has not been validated by Active Motif for this application. ChIP-validated antibodies are available for this target: Catalog No. 39535.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Purified IgG in 10 mM sodium phosphate ( pH 7.5), 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN4889658)

Nolan, Franco, Hance, Hayward, Isaacs: "Tumor-secreted Hsp90 subverts polycomb function to drive prostate tumor growth and invasion." in: The Journal of biological chemistry, Vol. 290, Issue 13, pp. 8271-82, (2015) (PubMed).

Papazyan, Voronina, Chapman, Luperchio, Gilbert, Meier, Mackintosh, Shabanowitz, Tackett, Reddy, Coyne, Hunt, Liu, Taverna: "Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis." in: eLife, Vol. 3, pp. e02996, (2014) (PubMed).

Ivanov, Pawlikowski, Manoharan, van Tuyn, Nelson, Rai, Shah, Hewitt, Korolchuk, Passos, Wu, Berger, Adams: "Lysosome-mediated processing of chromatin in senescence." in: The Journal of cell biology, Vol. 202, Issue 1, pp. 129-43, (2013) (PubMed).

Rada-Iglesias, Bajpai, Prescott, Brugmann, Swigut, Wysocka: "Epigenomic annotation of enhancers predicts transcriptional regulators of human neural crest." in: Cell stem cell, Vol. 11, Issue 5, pp. 633-48, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522