ATG5 antibody

Catalog No. ABIN4901026

This Mouse Monoclonal antibody specifically detects ATG5 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATG5 antibody (ABIN4901026)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ATG5 Antibody

Purpose: ATG5 is a protein encoded by the ATG5 gene which is approximately 32,4 kDa. ATG5 is localised to the cytoplasm. It is involved in the autophagy pathway, longevity regulating pathway, the innate immune system and pink/parkin mediated mitophagy. It functions as an E1-like activating enzyme, in combination with autophagy protein 12, in a ubiquitin-like conjugating system. It is involved in multiple cellular processes such as mitochondrial quality control after oxidative damage, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. ATG5 is expressed ubiquitously in human tissues and mutations in the ATG5 gene may result in Bardet-Biedl syndrome. STJ98903 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody detects endogenous ATG5 protein.

Specificity: The antibody detects endogenous ATG5 protein.

Characteristics: Mouse Monoclonal to ATG5.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Recombinant peptide derived from ATG5.

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Molecular Weight: 55kDa

Gene ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy