Caspase 1 antibody (Internal Region)

Catalog No. ABIN4901137

This Rabbit Polyclonal antibody specifically detects Caspase 1 in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for Caspase 1 antibody (Internal Region) (ABIN4901137)

Target: Caspase 1 (CASP1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caspase 1 Antibody

Binding Specificity: Internal Region

Purpose: Caspase-1 is a protein encoded by the CASP1 gene which is approximately 45,1 kDa. Caspase-1 is localised to the cytoplasm. It is involved in amyotrophic lateral sclerosis, toll-like receptor signalling pathways, p53 signalling and the TNFR1 pathway. This protein falls under the the cysteine-aspartic acid protease family. upon activation It plays a central role in the execution-phase of cell apoptosis. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules. Caspase-1 is expressed mainly in the spleen and lung. Mutations in the CASP1 gene may result in shigellosis and cowpox. STJ98574 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Caspase-1.

Specificity: Caspase-1 Polyclonal Antibody detects endogenous levels of Caspase-1

Characteristics: Rabbit Polyclonal to Caspase-1.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from Caspase-1

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1

Gene ID: 834

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome