DDIT3 antibody (AA 10-90)

Catalog No. ABIN4901264

The Mouse Monoclonal anti-DDIT3 antibody is suitable to detect DDIT3 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for DDIT3 antibody (AA 10-90) (ABIN4901264)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2B1

Product Details anti-DDIT3 Antibody

Binding Specificity: AA 10-90

Purpose: CHOP is a protein encoded by the DDIT3 gene which is approximately 19,1 kDa. CHOP is localised to the cytoplasm and nucleus. It is involved in the unfolded protein response, the MAPK signalling pathway and apoptosis modulation and signalling. This protein falls under the CCAAT/enhancer-binding protein family of transcription factors. It functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP and preventing their DNA binding activity. It is activated by endoplasmic reticulum stress and promotes apoptosis. CHOP is expressed in the muscle, nervous system, eye, liver and heart. Mutations in the DDIT3 gene may result in myxoid liposarcoma. STJ97774 was developed from clone 2B1 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of DDIT3

Specificity: CHOP Mouse Monoclonal Antibody (2B1) detects endogenous levels of DDIT3

Characteristics: Mouse Monoclonal to CHOP.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: synthetic peptide derived from CHOP

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-2000
IHC 1:100-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: CHOP

Gene ID: 1649

UniProt: P35638

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis