EpCAM antibody

Catalog No. ABIN490139

The Mouse Monoclonal anti-EpCAM antibody has been validated for WB, FACS, IP and ICC. It is suitable to detect EpCAM in samples from Human.

Quick Overview for EpCAM antibody (ABIN490139)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 323-A3

Product Details anti-EpCAM Antibody

Specificity: The antibody 323/A3 recognizes CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.

Purification: Protein A purified

Immunogen: Human breast cancer MCF-7 cells.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, ICC, IP, WB

Usage: Immunoprecipitation: Recommended dilution - 1-4 μg / ml per 100-500 μg of protein in 1 mL lysate. Western Blotting: Recommended dilution - 1-4 μg/mL. Positive control - A-431, MCF-7, SW480 cell lines. Immunohistochemistry (paraffin sections): Recommended

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: ESA / EPCAM

Background: Name/Gene ID: EPCAM

Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1

Gene ID: 4072

UniProt: P16422