Luciferase antibody

Catalog No. ABIN4901900

The Mouse Monoclonal anti-Luciferase antibody has been validated for WB and IHC (p). It is suitable to detect Luciferase in samples from Firefly.

Quick Overview for Luciferase antibody (ABIN4901900)

Target: Luciferase

Reactivity: Firefly

Host: Mouse

Clonality: Monoclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6B8

Product Details anti-Luciferase Antibody

Purpose: Luciferase or Luciferin 4-monooxygenase is a protein encoded by the luc gene which is produced in Photinus pyralis or the common eastern firefly.. Luciferase is localised to the peroxisome and is involved in chaperone binding. This protein falls under the ATP-dependent AMP-binding enzyme family and is one of the most extensively studied of the enzymes that catalyse light production in bioluminescent organisms and requires ATP and 02 as substrates. There are two reactions catalysed by luciferase, the first being the formation of an enzyme-bound luciferyl-adenylate and a second in which the luciferyl-adenylate undergoes an oxidative decarboxylation which results in the production of CO2, oxyluciferin, AMP, and green light with a wavelength of 562 nm. STJ97752 was developed from clone 6B8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of Luciferase.

Specificity: Luciferase Mouse Monoclonal Antibody (6B8) detects endogenous levels of Luciferase

Characteristics: Mouse Monoclonal to Luciferase.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Recombinant peptide derived from Luciferase

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-2000
IHC 1:100-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for Luciferase

Target: Luciferase

UniProt: P08659