RAD51 antibody

Catalog No. ABIN4902204

This anti-RAD51 antibody is a Mouse Monoclonal antibody detecting RAD51 in WB and ELISA. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for RAD51 antibody (ABIN4902204)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 4H10-G5-H4

Product Details anti-RAD51 Antibody

Purpose: Rad51 is a protein encoded by the RAD51 gene which is approximately 35 kDa. Rad51 is localised to the nucleus, cytoplasm and mitochondrion matirx. It is involved in the resolution of D-loop structures through synthesis-dependent strand annealing, DNA double-strand break repair, ATM pathway and the BRCA1 pathway. It is known to be involved in the homologous strand exchange, a key step in DNA repair through homologous recombination and repair of DNA. It catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Rad51 is highly expressed in the testis and thymus and less expressed in the small intestine, placenta, colon, pancreas and ovary. Mutations in the RAD51 gene result in breast cancer and Fanconi anemia, a disorder affecting all bone marrow elements. STJ99103 was developed from clone 4H10-G5-H4. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and detects endogenous levels of Rad51 and does not cross-react with related proteins.

Specificity: This antibody detects endogenous levels of Rad51 and does not cross-react with related proteins.

Characteristics: Mouse Monoclonal to Rad51.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Recombinant peptide derived from human Rad51.

Isotype: IgG2a

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: Rad51

Molecular Weight: 37kDa

Gene ID: 5888

UniProt: Q06609

Pathways: DNA Damage Repair