RHEB antibody

Catalog No. ABIN4902224

The Rabbit Polyclonal anti-RHEB antibody has been validated for WB and ELISA. It is suitable to detect RHEB in samples from Human, Mouse, Rat and Cow.

Quick Overview for RHEB antibody (ABIN4902224)

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHEB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RHEB Antibody

Purpose: RHEB is a protein encoded by the RHEB gene which is approximately 20,5 kDa. RHEB is localised to the cytoplasm, Golgi apparatus membrane, cytosol and endoplasmic reticulum membrane. It is involved in the regulation of lipid metabolism, insulin signalling-generic cascades, mTOR signalling, RET signalling, AMP-activated protein kinase signalling and the phospholipase D signalling pathway. The RHEB gene is a member of the small GTPase superfamily and encodes the lipid-anchored, cell membrane protein, RHEB. This protein is vital in regulation of growth and cell cycle progression. RHEB is ubiquitous expressed with highest levels observed in skeletal and cardiac muscle. Mutations in the RHEB can result in tuberous sclerosis. STJ98588 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody binds to endogenous levels of RHEB.

Specificity: RHEB Polyclonal Antibody detects endogenous levels of RHEB

Characteristics: Rabbit Polyclonal to RHEB.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from synthetic peptide derived from RHEB.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for RHEB

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Alternative Name: RHEB

Gene ID: 6009

UniProt: Q15382

Pathways: RTK Signaling