SIRT2 antibody

Catalog No. ABIN4902262

This Mouse Monoclonal antibody specifically detects SIRT2 in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SIRT2 antibody (ABIN4902262)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 1D4-H11-H11

Product Details anti-SIRT2 Antibody

Purpose: Mouse monoclonal to SirT2.

Specificity: This antibody detects endogenous levels of SirT2 and does not cross-react with related proteins.

Characteristics: Mouse Monoclonal to SirT2.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Purified recombinant human SirT2 protein expressed in E.coli.

Isotype: IgG2b

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Isoform 1 is expressed in heart, liver and skeletal muscle, weakly expressed in the cortex. Isoform 2 is strongly expressed in the cortex, weakly expressed in heart and liver. Weakly expressed in several malignancies including breast, liver, brain, kidney and prostate cancers compared to normal tissues. Weakly expressed in glioma cell lines compared to normal brain tissues (at protein level). Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SirT2

Molecular Weight: 43kDa

Gene ID: 22933

UniProt: Q8IXJ6