APEX1 antibody

Catalog No. ABIN4906178

The Mouse Monoclonal anti-APEX1 antibody has been validated for WB and IHC (p). It is suitable to detect APEX1 in samples from Human and Rat.

Quick Overview for APEX1 antibody (ABIN4906178)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 15C2

Product Details anti-APEX1 Antibody

Cross-Reactivity: Human, Rat

Purification: Purified by Protein G.

Immunogen: This APEX1 antibody is generated from a mouse immunized with a recombinant protein of human APEX1.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1

Background:

Synonyms: APE, APX, APE1, APEN, APEX, HAP1, REF1, DNA-(apurinic or apyrimidinic site) lyase, APEX nuclease, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, APEX1

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene, all encode the same protein. [provided by RefSeq, Jul 2008]

Gene ID: 328

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus