BBS4 antibody

Catalog No. ABIN4906487

The Mouse Monoclonal anti-BBS4 antibody has been validated for WB. It is suitable to detect BBS4 in samples from Human, Mouse and Rat.

Quick Overview for BBS4 antibody (ABIN4906487)

Target: BBS4 (Bardet-Biedl Syndrome 4 (BBS4))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BBS4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2C8

Product Details anti-BBS4 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This BBS4 antibody is generated from a mice immunized with a recombinant protein between 1-240 amino acids from human BBS4.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for BBS4

Target: BBS4 (Bardet-Biedl Syndrome 4 (BBS4))

Alternative Name: BBS4

Background:

Synonyms: Bardet-Biedl syndrome 4 protein, BBS4

Background: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome.

Gene ID: 585

UniProt: Q96RK4

Pathways: Hedgehog Signaling, Tube Formation, Maintenance of Protein Location