IRF3 antibody
Quick Overview for IRF3 antibody (ABIN4911171)
Target
See all IRF3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Cross-Reactivity
- Human
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Cross-Reactivity (Details)
- African Green Monkey
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Purification
- Purified by Protein G.
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Immunogen
- This IRF3 antibody is generated from a mouse immunized with a recombinant protein.
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Isotype
- IgG1
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Application Notes
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WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IF() -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 μg/μL
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Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 12 months
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- IRF3 (Interferon Regulatory Factor 3 (IRF3))
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Alternative Name
- IRF3
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Background
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Synonyms: IIAE7, Interferon regulatory factor 3, IRF-3, IRF3
Background: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
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Gene ID
- 3661
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UniProt
- Q14653
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Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Target
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