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IRF3 antibody

This anti-IRF3 antibody is a Mouse Monoclonal antibody detecting IRF3 in WB, IF, IHC (p), FACS and IF (cc). Suitable for Human and Green Monkey.
Catalog No. ABIN4911171

Quick Overview for IRF3 antibody (ABIN4911171)

Target

See all IRF3 Antibodies
IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity

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Human, Green Monkey

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This IRF3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Clone

2C4
  • Cross-Reactivity

    Human

    Cross-Reactivity (Details)

    African Green Monkey

    Purification

    Purified by Protein G.

    Immunogen

    This IRF3 antibody is generated from a mouse immunized with a recombinant protein.

    Isotype

    IgG1
  • Application Notes

    WB 1:300-5000
    FCM 1:20-100
    IHC-P 1:200-400
    IF(ICC) 1:50-200
    IF()

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Expiry Date

    12 months
  • Target

    IRF3 (Interferon Regulatory Factor 3 (IRF3))

    Alternative Name

    IRF3

    Background

    Synonyms: IIAE7, Interferon regulatory factor 3, IRF-3, IRF3

    Background: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

    Gene ID

    3661

    UniProt

    Q14653

    Pathways

    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
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