NOS1 antibody (pSer1417)

Catalog No. ABIN4912434

This anti-NOS1 antibody is a Rabbit Polyclonal antibody detecting NOS1 in WB, ELISA, ICC, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human, Rat and Xenopus laevis.

Quick Overview for NOS1 antibody (pSer1417) (ABIN4912434)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NOS1 Antibody

Binding Specificity: pSer1417

Cross-Reactivity: Human, Rat, Xenopus laevis

Predicted Reactivity: Mouse,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human nNOS around the phosphorylation site of Ser1417

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: nNOS

Background:

Synonyms: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, CASP9

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Gene ID: 842

UniProt: P55211

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction