RPA2 antibody

Catalog No. ABIN4913599

This Mouse Monoclonal antibody specifically detects RPA2 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for RPA2 antibody (ABIN4913599)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5C3

Product Details anti-RPA2 Antibody

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: This RFA2 antibody is generated from a mouse immunized with a recombinant protein of human RFA2.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RFA2

Background:

Synonyms: REPA2, RPA32, RP-A p32, RP-A p34, Replication protein A 32 kDa subunit, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RPA2, RPA34

Background: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Gene ID: 6118

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA