UCHL1 antibody

Catalog No. ABIN4915017

This anti-UCHL1 antibody is a Mouse Monoclonal antibody detecting UCHL1 in WB, IF and IHC (p). Suitable for Human and Mouse.

Quick Overview for UCHL1 antibody (ABIN4915017)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3C2

Product Details anti-UCHL1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This UCHL1 monoclonal antibody is generated from mouse immunized with UCHL1 recombinant protein.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1

Background:

Synonyms: NDGOA, PARK5, PGP95, PGP9.5, Uch-L1, HEL-117, PGP 9.5, HEL-S-53, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Neuron cytoplasmic protein 9.5, Ubiquitin thioesterase L1, UCHL1

Background: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Gene ID: 7345

UniProt: P09936

Pathways: Feeding Behaviour