EGFR antibody (pTyr869) (FITC)

Catalog No. ABIN492341

The Mouse Monoclonal anti-EGFR antibody is suitable to detect EGFR in samples from Human and Mouse. It has been validated for IF.

Quick Overview for EGFR antibody (pTyr869) (FITC) (ABIN492341)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is conjugated to FITC

Application: Immunofluorescence (IF)

Clone: 12A3

Product Details anti-EGFR Antibody (FITC)

Binding Specificity: pTyr869

Specificity: Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031FC-N does not crossreact with the highly homologues pTyr877 of activated erbB2.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Characteristics: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Immunogen: Phosphopeptide conjugated to KLHAA Sequence: E K E pY H A ERemarks: Epitope: Phosphotyrosine 869

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry: Use at 1-10 μg/mLUse ABIN126761 or ABIN126759 for Immunoblotting and Immunoprecipitationapplications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide / PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Aiquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. ThawedAliquots may be stored at 4 °C up to 3 months.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR / ERBB1

Background: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins