LY9 antibody

Catalog No. ABIN492431

The Mouse Monoclonal anti-LY9 antibody is suitable to detect LY9 in samples from Human. It has been validated for WB, IP, FACS, IF and Func.

Quick Overview for LY9 antibody (ABIN492431)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY9 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: HLy9-25

Product Details anti-LY9 Antibody

Specificity: The antibody recognizes CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

Cross-Reactivity (Details): Species reactivity (tested):Human

Characteristics: Synonyms: Ly-9, T-lymphocyte surface antigen Ly-9, Lymphocyte antigen 9

Purification: Protein A affinity chromatography

Purity: > 95 % by SDS-PAGE

Immunogen: CD299-transfected 300. 19 pre-B cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Use peripheral blood lymphocytes as positive control. Immunoprecipitation. Western blot. Immunocytochemistry. Functional Application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: CD229 / LY9

Background: CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9

Gene ID: 4063

UniProt: Q9HBG7

Pathways: BCR Signaling