APAF1 antibody (C-Term)

Catalog No. ABIN492509

The Mouse Monoclonal anti-APAF1 antibody has been validated for IHC (p) and EIA. It is suitable to detect APAF1 in samples from Human, Mouse and Rat.

Quick Overview for APAF1 antibody (C-Term) (ABIN492509)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 5-00E-011

Product Details anti-APAF1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes APAF1.

Characteristics: Synonyms: Apaf-1, KIAA0413, Apoptotic protease-activating factor 1

Purification: Immunoaffinity Chromotography

Immunogen: A peptide corresponding to amino acids near the carboxy terminus of Human Apaf1.

Isotype: IgG

Application Details

Application Notes: ELISA. Immunohistochemistry: 5-10 μg/mLPositive Control: K562 Whole Cell Lysate
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: APAF1

Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 transcript is ubiquitously expressed in human tissues (1).Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413

Gene ID: 317

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity