PTK6 antibody

Catalog No. ABIN492612

The Mouse Monoclonal anti-PTK6 antibody (Clone 24B7) (ABIN492612) specifically detects PTK6 in WB. The antibody is reactive with Human samples.

Quick Overview for PTK6 antibody (ABIN492612)

Target: PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTK6 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 24B7

Product Details anti-PTK6 Antibody

Specificity: Recognizes BRK/PTK6

Characteristics: Synonyms: Tyrosine-protein kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Immunogen: Peptide conjugated to hemocyanin. Epitope: Internal peptide derived from BRK/PTK6 sequence

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Potitive Control: Cell lysate from untreated MDA-MB 468 cells (See Protocols formore details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control: Cell lysate from untreated MDA-MB-468 cells, breast adenocarcinoma(Human)Format: Lyophilized cell lysate from serum starved MDA-MB-468 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide, PEG, Sucrose and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody in aliquots) at -20 °C. Avoid repeated freeze-thaw cycles.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PTK6

Target: PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Alternative Name: PTK6 / BRK

Background: This protein encoded is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation.Synonyms: Breast tumor kinase, Tyrosine-protein kinase 6, Tyrosine-protein kinase BRK

Gene ID: 5753

UniProt: Q13882