PAK4 antibody

Catalog No. ABIN492621

This anti-PAK4 antibody is a Mouse Monoclonal antibody detecting PAK4 in WB. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for PAK4 antibody (ABIN492621)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 6C1

Product Details anti-PAK4 Antibody

Specificity: Recognizes p21 activated kinase 4 (PAK4).

Characteristics: Synonyms: PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, Serine/threonine-protein kinase PAK 4

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Peptide conjugated to hemocyaninEpitope: Peptide derived from the kinase activation loop.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from untreated SW480 cellsDescription: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4

Background: P21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.Synonyms: KIAA1142, PAK 4, PAK-4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4

Gene ID: 10298

UniProt: O96013

Pathways: RTK Signaling