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Recombinant Calponin antibody

The Rabbit Monoclonal anti-Calponin antibody has been validated for IHC (p), IF and FACS. It is suitable to detect Calponin in samples from Human and Rat.
Catalog No. ABIN4949269

Quick Overview for Recombinant Calponin antibody (ABIN4949269)

Target

Calponin

Antibody Type

Recombinant Antibody

Reactivity

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Human, Rat

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This Calponin antibody is un-conjugated

Application

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone

CNN1-1408R
  • Purification

    Protein A affinity chromatography

    Immunogen

    Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.

    Isotype

    IgG kappa
  • Application Notes

    Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10^6 cells,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Preservative

    Azide free

    Storage

    4 °C,-20 °C

    Storage Comment

    Store the recombinant Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    Calponin

    Background

    Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
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