Recombinant CD99 antibody

Catalog No. ABIN4949314

The Rabbit Monoclonal anti-CD99 antibody has been validated for FACS, IF and IHC (p). It is suitable to detect CD99 in samples from Human.

Quick Overview for Recombinant CD99 antibody (ABIN4949314)

Target: CD99

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MIC2-1495R

Product Details anti-CD99 Antibody

Purification: Protein A affinity chromatography

Immunogen: Human protein was used as the immunogen for the recombinant CD99 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant CD99 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 2-4 μg/million cells in 0.1ml,Immunofluorescence: 2-4 μg/mL,Immunohistochemistry (FFPE): 5-10 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background: CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]