Recombinant EpCAM antibody (AA 20-60)

Catalog No. ABIN4949336

This anti-EpCAM antibody is a Rabbit Monoclonal antibody detecting EpCAM in WB, FACS, IF and IHC (p). Suitable for Human.

Quick Overview for Recombinant EpCAM antibody (AA 20-60) (ABIN4949336)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EGP40-1555R

Product Details anti-EpCAM Antibody

Binding Specificity: AA 20-60

No Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Purification: Protein A affinity chromatography

Immunogen: A portion of amino acids 20-60 was used as the immunogen for the recombinant EpCAM antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,IF: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background: Recognizes a 40-43  kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.