Recombinant Glypican 3 antibody

Catalog No. ABIN4949349

This Rabbit Monoclonal antibody specifically detects Glypican 3 in IF, FACS and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for Recombinant Glypican 3 antibody (ABIN4949349)

Target: Glypican 3 (GPC3)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GPC3-1534R

Product Details anti-Glypican 3 Antibody

Purification: Protein A affinity chromatography

Immunogen: A partial human protein was used as the immunogen for the recombinant Glypican-3 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Glypican-3 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Glypican-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: Glypican 3 / GPC3

Background: Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt]

Pathways: Glycosaminoglycan Metabolic Process