Recombinant Nkx2-2 antibody

Catalog No. ABIN4949369

This anti-Nkx2-2 antibody is a Rabbit Monoclonal antibody detecting Nkx2-2 in IF, FACS and IHC (p). Suitable for Human.

Quick Overview for Recombinant Nkx2-2 antibody (ABIN4949369)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NX2-1422R

Product Details anti-Nkx2-2 Antibody

Purification: Protein A affinity chromatography

Immunogen: A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant NKX2.2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells in 0.1ml,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

Gene ID: 4821

Pathways: Dopaminergic Neurogenesis