Aurora Kinase B antibody (AA 89-251)

Catalog No. ABIN4949412

This Mouse Monoclonal antibody specifically detects Aurora Kinase B in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Aurora Kinase B antibody (AA 89-251) (ABIN4949412)

Target: Aurora Kinase B (AURKB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AURKB-1521

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: AA 89-251

Purification: Protein G affinity

Immunogen: Amino acids 89-251 were used as the immunogen for this Aurora B antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the Aurora B antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/10e6 cells in 0.1ml,IF: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Aurora B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: Aurora B / AURKB

Background: Recognizes a protein of 39-45  kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades