Calnexin antibody

Catalog No. ABIN4949485

The Mouse Monoclonal anti-Calnexin antibody has been validated for WB, ELISA, IHC (p) and IF/ICC. It is suitable to detect Calnexin in samples from Human.

Quick Overview for Calnexin antibody (ABIN4949485)

Target: Calnexin (CANX)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (fixed cells) (IF/ICC)

Clone: CANX-1541

Product Details anti-Calnexin Antibody

No Cross-Reactivity: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: A partial human recombinant protein was used as the immunogen for the Calnexin antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Titering of the Calnexin antibody may be required for optimal performance.\. ELISA: 2-4 μg/mL (order BSA-free format for coating),WB: 1-2 μg/mL,IF/ICC: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Calnexin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background: It recognizes a protein of 90  kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Pathways: MAPK Signaling, Thyroid Hormone Synthesis