CD3 epsilon antibody (Cytoplasmic Domain)

Catalog No. ABIN4949516

The Mouse Monoclonal anti-CD3 epsilon antibody (Clone PC3-188A) (ABIN4949516) specifically detects CD3 epsilon in FACS, WB and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CD3 epsilon antibody (Cytoplasmic Domain) (ABIN4949516)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Grade: Carrier-free

Clone: PC3-188A

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 156-168, Cytoplasmic Domain

Purpose: CD3e Antibody / Cytoplasmic domain (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the CD3e antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway