GJB1 antibody (Cytoplasmic Loop)
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- Target See all GJB1 Antibodies
- GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))
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Binding Specificity
- Cytoplasmic Loop
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Reactivity
- Human, Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GJB1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purification
- Protein G affinity chromatography
- Immunogen
- Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).
- Clone
- M12-13
- Isotype
- IgG
- Top Product
- Discover our top product GJB1 Primary Antibody
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- Application Notes
- Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL for 30 min at RT
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))
- Alternative Name
- Connexin 32 / GJB1 (GJB1 Products)
- Synonyms
- GJIC antibody, cmtx antibody, cmtx1 antibody, connexin-30 antibody, connexin32 antibody, cx30 antibody, cx32 antibody, gja1 antibody, CX32 antibody, GJB1 antibody, AI118175 antibody, Cnx32 antibody, Cx32 antibody, Gjb-1 antibody, CMTX antibody, CMTX1 antibody, gjb1-a antibody, gap junction protein beta 1 antibody, connexin 32 antibody, probable serine/threonine-protein kinase CST antibody, gap junction protein, beta 1 antibody, gap junction protein beta 1 L homeolog antibody, gjb1 antibody, CX32 antibody, LOC9303136 antibody, GJB1 antibody, Gjb1 antibody, gjb1.L antibody
- Background
- This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.
- Pathways
- Cell-Cell Junction Organization
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