Desmoglein 2 antibody

Catalog No. ABIN4949611

Product Details anti-Desmoglein 2 Antibody

Target: Desmoglein 2 (DSG2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Purification: Protein G affinity chromatography

Immunogen: A recombinant human DSG2 protein was used as the immunogen for the Desmoglein 2 antibody.

Clone: 6D8

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Desmoglein 2 antibody should be determined by the researcher.\. FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 1-2 μg/mL,Immunoprecipitation: suitable

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Desmoglein 2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Desmoglein 2

Target: Desmoglein 2 (DSG2)

Alternative Name: Desmoglein 2 / DSG2 (DSG2 Products)

Synonyms: DSG2 antibody, dsc antibody, dsga antibody, si:dkeyp-51f12.6 antibody, wu:fj23f02 antibody, ARVC10 antibody, ARVD10 antibody, CDHF5 antibody, CMD1BB antibody, HDGC antibody, AA408168 antibody, D18Ertd293e antibody, desmoglein 2 antibody, desmoglein 2, tandem duplicate 1 antibody, DSG2 antibody, dsg2.1 antibody, dsg2 antibody, Dsg2 antibody

Background: Recognizes a protein of 165  kDa, identified as Desmoglein-2 (DSG2). This monoclonal antibody recognizes the extracellular domain of human desmoglein-2. Desmoglein-2 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).

Pathways: Cell-Cell Junction Organization