Desmoglein 3 antibody (Extracellular Domain)

Catalog No. ABIN4949614

The Mouse Monoclonal anti-Desmoglein 3 antibody has been validated for WB, IF, FACS and IP. It is suitable to detect Desmoglein 3 in samples from Human.

Quick Overview for Desmoglein 3 antibody (Extracellular Domain) (ABIN4949614)

Target: Desmoglein 3 (DSG3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 5H10

Product Details anti-Desmoglein 3 Antibody

Binding Specificity: Extracellular Domain

Purification: Protein G affinity chromatography

Immunogen: A recombinant human protein corresponding to the extracellular portion of DSG3 was used as the immunogen for the Desmoglein 3 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Desmoglein 3 antibody should be determined by the researcher.\. WB: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunoprecipitation: suitable

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Desmoglein 3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Desmoglein 3

Target: Desmoglein 3 (DSG3)

Alternative Name: Desmoglein 3 / DSG3

Background: Recognizes a protein of 130  kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.