The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Preservative
Azide free
Storage
4 °C,-20 °C
Storage Comment
Store the DOG1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
DOG1 is expressed in the human gastrointestinal epithelium and is highly expressed in the gastrointestinal interstitial cells of Cajal, where it plays an important role in epithelial chloride secretion mediating intestinal motility. [Wiki]