EPO antibody

Catalog No. ABIN4949655

This Mouse Monoclonal antibody specifically detects EPO in WB, FACS, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for EPO antibody (ABIN4949655)

Target: EPO (Erythropoietin (EPO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPO antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EPO-1368

Product Details anti-EPO Antibody

Purification: Protein G affinity chromatography

Immunogen: A human partial recombinant protein was used as the immunogen for the EPO antibody.

Isotype: IgG

Application Details

Application Notes: Titering of the EPO antibody may be required for optimal performance.

1. Required HIER: boil tissue sections in 10  mM citrate buffer,  pH 6, for 10-20 min.\. WB: 0.5-1 μg/mL,IF: 1-2 μg/mL,Immunohistochemistry (FFPE): 4-8 μg/mL for 30 min at RT (1),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the EPO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: Erythropoietin / EPO

Target Type: Hormone

Background: Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity