JUP antibody (N-Term)

Catalog No. ABIN4949714

This anti-JUP antibody is a Mouse Monoclonal antibody detecting JUP in WB, IF and IP. Suitable for Human and Mouse.

Quick Overview for JUP antibody (N-Term) (ABIN4949714)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 11E4-

Product Details anti-JUP Antibody

Binding Specificity: N-Term

Purification: Protein G affinity chromatography

Immunogen: Recombinant full length human gamma Catenin fused to maltose binding protein was used as the immunogen for the Plakoglobin antibody. The epitope has been found to be in the N-terminal region.

Isotype: IgG1 kappa

Application Details

Application Notes: Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,Immunoprecipitation: suitable

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Plakoglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Gamma Catenin / Plakoglobin

Background: It recognizes a protein of 80-87  kDa, identified as gamma-Catenin or Plakoglobin. The catenins (alpha, beta, gamma and δ,) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. alpha T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. alpha T-catenin binds to alpha E-catenin as well as to beta-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for alpha-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location