Myeloid antibody
Quick Overview for Myeloid antibody (ABIN4949815)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purification
- Protein G affinity chromatography
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Immunogen
- Recombinant human protein was used as the immunogen for the Myeloid antibody.
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Isotype
- IgG1 kappa
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anti-Myeloid antibody
Reactivity: Human, Macaque FACS, IHC (p), IF Host: Mouse Monoclonal MYADM-972 unconjugated
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Application Notes
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Optimal dilution of the Myeloid antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Preservative
- Azide free
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store the Myeloid antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Myeloid
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Background
- Recognizes a myeloid associated differentiation antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of human and monkey myeloid cells. This mAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
Target
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