Thrombomodulin antibody (AA 69-194)

Catalog No. ABIN4949900

This anti-Thrombomodulin antibody is a Mouse Monoclonal antibody detecting Thrombomodulin in WB, ELISA, FACS, IF and IHC (p). Suitable for Human.

Quick Overview for Thrombomodulin antibody (AA 69-194) (ABIN4949900)

Target: Thrombomodulin (THBD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: THBD-1591

Product Details anti-Thrombomodulin Antibody

Binding Specificity: AA 69-194

Purification: Protein G affinity chromatography

Immunogen: Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT,ELISA: 2-4 μg/mL, order the BSA-free formulation for coating,WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Thrombomodulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: Thrombomodulin / CD141

Background: It recognizes a protein of 75  kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.