Villin 1 antibody (AA 179-311)

Catalog No. ABIN4949934

This Mouse Monoclonal antibody specifically detects Villin 1 in WB, IF, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Villin 1 antibody (AA 179-311) (ABIN4949934)

Target: Villin 1 (VIL1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: VIL1-1314

Product Details anti-Villin 1 Antibody

Binding Specificity: AA 179-311

Purification: Protein G affinity chromatography

Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Titration of the Villin antibody may be required for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.25-0.5 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: Villin

Background: Recognizes a protein of ~93  kDa which is identified as Villin (VIL1). It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization